ABSTRACT
Objective To evaluate the efficacy and safety of intense pulsed light (IPL) combined with hydroquinone cream for melasma treatment.Methods Thirty-five patients with melasma were treated between February 2015 and February 2017,20 patients treated with IPL combined with hydroquinone cream and 15 patients with IPL alone.All cases were female patients with Fitzpatrick skin types Ⅲ and Ⅳ,and subjected to 4 weeks treatment 1 time,5 times for a course of treatment.All patients were followed up for six months and the efficacy and safety of the treatment were analyzed comprehensively by subjective satisfaction and physician evaluation.Results All the patients were treated without discomfort and treated patients were effectively followed up.The satisfactory rate of IPL treatment group was 73.3％,and that of combined with hydroquinone cream group was 100％.Two groups of satisfactory rates were higher in IPL combined with hydroquinone cream.The total effective rate of the IPL treatment group was 53.3％ compared with that of the 35 patients before and after treatment;in IPL combined with hydroquinone cream treatment group the total effective rate was 85.0％.IPL combined with hydroquinone cream treatment group was significantly more effective than the treatment group (P＜0.05).All treatment patients did not present with tissue damage,pigmentation and other adverse reactions.Conclusions IPL combined with hydroquinone cream is a safe and effective treatment of melasma,and patients get a higher rate of satisfaction.
ABSTRACT
BACKGROUND: Dermal fibroblasts are widely used and demanded, and there are various isolation methods, but no comparative studies on enzyme digestion methods are reported. OBJECTIVE: To compare the cell count, morphology, migration and proliferation of human dermal fibroblasts isolated by two enzyme digestion methods. METHODS: Human dermal fibroblasts were isolated using either dispase-collagenase or trypsin, and their cell yield and viability were assessed by morphology, cell count and proliferation curve by cell counting-kit 8 assay. The ability of migration was observed by cell scratch test. RESULTS AND CONCLUSION: The fibroblasts digested with dispase-collagenase were fused at 6-7 days after inoculation, and the cells isolated by trypsin digestion were fused at 8-9 days after inoculation. Fibroblasts could be obtained by both two digestion methods. The production in the dispase-collagenase group was significantly higher than that in the trypsin group. The migration rate in the dispase-collagenase group was significantly faster than that in the trypsin group. The growth cures of the human dermal fibroblasts in the two groups revealed that the cell count was positively correlated with time, and the absorbance values of the dermal fibroblasts in the dispase-collagenase group were significantly higher than those in the trypsin group at 3, 4 and 5 days after incubation. To conclude, the cell yields, migration and proliferation of dermal fibroblasts digested with dispase-collagenase are significantly higher than those of the cells digested by trypsin, indicating that dispase-collagenase digestion results in better isolation and viability of dermal fibroblasts from the dermis.
ABSTRACT
OBJECTIVE:To study the antiasthmatic effect of Mabuterol in guinea pigs.METHODS:Severe dyspnea was induced by2%acetylcholine and0.1%histamine solution in conscious guinea pigs.RESULTS:Experiment showed that Mabuterol could significantly inhibit the experimental asthma induced by mixture of2%acetylcholine and0.1%histamine solution in conscious guinea pigs.It also significantly prolonged the latency of induced asthma and decreased the number of guinea pigs on twitches induced by asthma.Bronchodilating effect of Mabuterol was in a dose-dependent manner.The ED 50 of Mabuterol was0.2mg/kg and95%confidence limit was0.08～0.49mg/kg.CONCLUSION:In comparison with salbutanmol,Mabuterol is longer in duration and more potent in action of bronchodilatation.